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Extraction conditions for extraction yield ( Y   1  ), total phenolic content (TPC, Y   2  ), and inhibition rate in human gastric carcinoma cells ( Y   3  ) were optimized using response surface methodology for fermented Rhus verniciflua stokes (FRV). Optimal extraction conditions for effective use of FRV as a biofunctional material in food and pharmaceutical industries were investigated. Independent variables were extraction temperature ( X   1  =25-105°C), extraction time ( X   2  =4-20 h), and the solvent to solid ratio ( X   3  =10-30 mL/g). A second order polynomial model satisfactorily fitted experimental data with coefficient of determination ( R   2  ) values of 0.9613, 0.9851, and 0.8038, respectively, for Y   1  , Y   2  , and Y   3  . Optimal conditions for the highest extraction yield of 2.37%, a total phenol content (TPC) value of 113.75 mg of tannic acid equivalents (TAE)/g, and an inhibition rate of 72.61% were derived at X   1  =89.95°C, X   2  =9.25 h, and X   3  =25 mL/g.

Optimization of the extraction process for fermented Rhus verniciflua stokes using response surface methodology