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Characeae are closely related to the ancient algal ancestors of all land plants. The long characean cells display a pH banding pattern to facilitate inorganic carbon import in the acid zones for photosynthetic efficiency. The excess OH − , generated in the cytoplasm after CO 2  is taken into the chloroplasts, is disposed of in the alkaline band. To identify the transporter responsible, we searched the  Chara australis  transcriptome for homologues of mouse Slc4a11, which functions as OH − /H +  transporter. We found a single Slc4-like sequence CL5060.2 (named CaSLOT). When CaSLOT was expressed in  Xenopus  oocytes, an increase in membrane conductance and hyperpolarization of resting potential difference (PD) was observed with external pH increase to 9.5. These features recall the behavior of Slc4a11 in oocytes and are consistent with the action of a pH-dependent OH − /H +  conductance. The large scatter in the data might reflect intrinsic variability of CaSLOT transporter activation, inefficient expression in the oocyte due to evolutionary distance between ancient algae and frogs, or absence of putative activating factor present in  Chara  cytoplasm. CaSLOT homologues were found in chlorophyte and charophyte algae, but surprisingly not in related charophytes Zygnematophyceae or Coleochaetophyceae.

The molecular identity of the characean OH− transporter: a candidate related to the SLC4 family of animal pH regulators