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Under stress conditions, microalgae are known to accumulate large amounts of neutral lipids and carbohydrates, which can be used for biofuel production. However, on-line measurement of microalgal biochemical composition is a difficult task which makes the microalgal process rather difficult to manage. In this paper, we propose a so called adaptive interval observer for the on-line estimation of neutral lipid and carbohydrate quotas in microalgae. The observer is based on a change of coordinates that involves a time-varying gain. We introduce dynamics for the gain, whose trajectory converges toward a predefined optimal value (which maximizes the convergence rate of the observer). The observer performance is illustrated with experimental data of Isochrysis sp. cultures under nitrogen limitations and day-night cycle. The proposed observer design appears to be a suitable robust estimation technique.

Estimation of neutral lipid and carbohydrate quotas in microalgae using adaptive interval observers