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Intramedullary spinal cord neoplasms represent 2–4 % of central nervous system tumors, of which astrocytic gliomas represent 80 %. Patients presenting with spinal cord astrocytomas span the traditional pediatric and adult age divisions, having an overall age-distribution that is younger than cohorts with supratentorial gliomas. WHO grade I and II astrocytomas have better outcomes that are largely dependent on extent of surgical resection [10], whereas Grade III and IV astrocytomas are less amenable to safe surgical resection, and typically require adjuvant radiation and chemotherapy for treatment. Given the premium on preserving neurologic function during spinal cord surgery, intraoperative frozen section histologic analysis has an important role in driving therapeutic decision-making. However, histologic grading can be challenging in spinal cord astrocytomas because of the often relatively small samples obtained at the time of the surgical procedure. Therefore, grade-defining molecular biomarkers would be particularly useful for the accurate diagnostic classification of these tumors [13]. Recent genome level sequencing studies of supratentorial gliomas revealed discrete genomic alterations that discriminate pilocytic astrocytomas, WHO grade II and III diffuse gliomas, and WHO grade IV glioblastoma (GBM), with notable differences between pediatric [9, 14, 15, 20] and adult [2, 3, 6] patients. To address the hypothesis that genomic alterations could segregate spinal cord astrocytoma histologic grades, we performed sequencing of cancer-related genes in a cohort of 17 tumors.Spinal cord astrocytomas from children and adults were obtained as formalin-fixed, paraffin-embedded (FFPE) specimens from Massachusetts General Hospital, the University of Toronto, and New York University. Central neuropathology review performed by a neuropathologist (DNL) and specimens with clear histologic diagnosis and grading were used for further analysis. The characteristics of the discovery cohort (n = 17 specimens) are listed in Table 1. Targeted sequencing of 560 cancer related genes and 39 translocation events was performed on DNA extracted from these specimens (Supplementary Table 1) [5]. Briefly, DNA was sonicated to achieve an average fragment size of 250 base pairs, size selected and barcoded. Multiplexed pools were hybridized with biotinylated baits (Agilent SureSelect) designed to capture exonic sequences. The captures were sequenced on the Illumina HiSeq 2500 in Rapid Run Mode. Mutation analysis was performed by MuTect [4] and SomaticIndelDetector, copy number variant analysis was performed by ReCapSeg, and rearrangement analysis was performed by BreaKmer [1]. When applicable, statistical comparisons were performed by Chi squared test.Table 1Baseline characteristics of discovery cohortThe most recurrent findings in Grade I spinal cord astrocytomas were a BRAF-KIAA1549 translocation (n = 3/10) and BRAF copy number gain (n = 5/10) (Fig. 1). Additionally, WHO grade I astrocytomas were found to have non-synonymous mutations in NF2, NTRK1, NTRK3, PDGFRA, and TP53 (Supplementary Table 2). WHO grade II astrocytomas were similarly characterized by alterations involved in the MAPK-ERK or PI3K pathways, including BRAF-KIAA1549 translocation (n = 1/3) and BRAF amplification (n = 2/3). For samples with sufficient material, low coverage whole genome sequencing (mean 1× depth) was performed revealing that the BRAF amplification resulted from a chromosome 7 arm level gain in three of these specimens (SA-N101, SA-TL07, and SA-TL17, Supplementary Figure 1). Notably, no specimen in the discovery cohort was characterized by the BRAF V600E mutation.Fig. 1Exomic characterization of spinal cord astrocytomas reveals that BRAF alterations and the H3F3A K27M mutation segregate histologic grade. Central neuropathology review was performed on the cohort of specimens used in this study (top row). Grade I and ...In addition, we observed that all four Grade III and IV astrocytomas in the discovery cohort shared the H3F3A K27M mutation. Further targeted Sanger sequencing of H3F3A was performed in five additional specimens (validation cohort) and revealed the K27M mutation in 2/3 spinal Grade IV astrocytomas and 0/2 Grade I astrocytomas (Supplementary Figure 2). The age distribution of our findings are consistent with prior observations that H3F3A K27M primarily occurs in pediatric and young adult gliomas [11, 15, 19]. In the aggregate cohort of 22 specimens (discovery and validation cohorts), the presence of H3F3A K27M in Grade III and IV (85.7 %, n = 6/7 specimens) and absence in Grade I and II (n = 0/15 specimens) astrocytomas was a statistically significant difference (p u003c 0.001, Chi squared test with Yates correction).Of note, while variants in IDH1 and IDH2 were noted in four specimens (Supplementary Table 2), none of these represented the recurrent mutations previously described in adult glioma. Loss of heterozygosity analysis of variant allele frequency [17] did not reveal co-deletion of chromosomes 1p and 19q (Supplementary Figure 3), further confirming that the tumors analyzed in the discovery cohort were astrocytic.The distribution of mutations observed may partially underlie the well-established demographic differences between patients with spinal cord gliomas compared to their supratentorial counterparts. For instance, whole genome analyses of pediatric intracranial gliomas have been reported recently with convergence of alterations in Grade I and II gliomas on MAPK-ERK and PI3K pathways [9]. Pediatric high grade gliomas, on the other hand, have been characterized by recurrent mutations in chromatin remodeling genes H3F3A, ATRX, and DAXX in 44 % of sequenced tumors [15]. Similarly, seminal work revealed that H3F3A K27M is found in 71 % of pediatric diffuse intrinsic pontine glioma, the presence of which correlated with worse outcomes [11]. Across pediatric and young adult GBM, H3F3A K27M mutations occur mutually exclusive of other category-defining recurrent mutations (such as mutations in IDH1 and TERT promoter) and are found predominantly in midline lesions bearing the transcriptomic profile of the proneural GBM subtype [18]. A recent report noted positive H3F3A K27M immunohistochemical staining in 11 spinal glioblastomas, 3 anaplastic astrocytomas, and 2 anaplastic gangliogliomas [7]. Together with our observation of H3F3A K27M occurring in 86 % of Grade III and IV spinal cord astrocytomas, this supports the concept of a shared teleology between aggressive astrocytic gliomas arising in midline structures of the craniospinal axis. Future transcriptional analysis of spinal cord astrocytomas can assess whether these lesions share similar changes noted in H3F3A K27M mutant supratentorial gliomas.The BRAF alterations in a high percentage of WHO grade I and II spinal cord astrocytomas point towards a potential therapeutic approach, as BRAF–MEK inhibitors have demonstrated success in BRAF-mutant cancer types. Accordingly, targeting the BRAF–MEK pathway in pediatric gliomas is under active evaluation [12]. Our findings suggest that patients with spinal cord astrocytomas could be considered for enrollment in clinical trials targeting these pathways. From a surgical management standpoint, the hotspot H3F3A K27M mutation has the potential to be genotyped within an intraoperative timeframe, to guide the aggressiveness of surgical resection by balancing the neuromonitoring-based potential for postoperative neurologic deficit with the predicted natural history defined by H3F3A K27M mutation status [16]. Detection of this mutation could ultimately guide novel adjuvant treatment strategies, as inhibition of histone deacetylase and histone demethylase has demonstrated in vivo efficacy in xenografts of H3F3A K27M mutant gliomas [8].While our findings do not indicate alterations specific to spinal cord astrocytomas versus supratentorial disease, larger cohort studies performing deep coverage whole genome or transcriptome may reveal unique copy number alterations or translocations in these infiltrative tumors. In summary, the findings described here indicate that BRAF alterations and histone H3F3A K27M mutations are grade-related features of spinal cord astrocytomas that should enter routine initial evaluation of spinal cord gliomas, and provide a potential foundation for adjuvant therapeutic strategies.

BRAF alteration status and the histone H3F3A gene K27M mutation segregate spinal cord astrocytoma histology