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Patches of surface-immobilized and catalytically active enzyme, immersed into a solution with the corresponding substrate, induce flow in the solution. Such systems are currently investigated as a promising direction in the development of self-powered micropumps that could operate autonomously within microfluidic devices. Here, we investigate the influence of confinement, within a slab of height  H , on the response exhibited by silica tracer particles sedimented near a chemically active glucose oxidase patch which is immersed into a glucose solution of very low ionic strength. Irrespective of the value  H , within the range explored in this study, a region depleted of tracers forms around the patch. When  H  is not much larger than the radius of the patch, the rate of growth of the depletion zone depends on  H ; somewhat surprisingly, this dependence is influenced by the glucose concentration. The results are discussed within the context of a simple model for a chemically active patch.

The impact of geometrical confinement in a slab on the behavior of tracer particles near active glucose oxidase micropump