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This paper examines the influence of frozen storage over 34 weeks on the rheological properties as well as the chemical and microstructural characteristics of gels made from muscle of blue whiting (Micromesistius poutassou) subjected to different gelling treatments entailing three combinations of pressure, temperature and time: 200 MPa, < 10 °C, 10 min (lot L), 375 MPa, 38 °C, 20 min (lot H) and atmospheric pressure, 37 °C, 30 min and then 90 °C, 50 min (lot T). Freezing at -40 °C caused certain changes in rheological parameters. In heat-induced gels, breaking deformation, elasticity and cohesiveness increased. Of the high-pressure-induced gels, breaking force increased and cohesiveness decreased in the gel formed at lower pressures, while the only change in the gel formed at higher pressure was some loss of elasticity. There was a general fall in water holding capacity (WHC) values. Lightness remained stable. In terms of protein solubility, there was an increase in covalent bonds in lot L. As for the ultrastructure, all gels matrixes were more disorganized as a result of freezing. In the course of frozen storage, the greatest changes in rheological parameters generally took place during the first 8 weeks, and in all the gels there was a decrease in WHC. In the heat-induced gel the changes were less marked over the storage period compared with those in the high-pressure-induced gels, but the heat-induced gel was more brittle and did not maintain maximum folding test scores. Covalent bonds increased and hydrophobic interactions decreased in all lots. The general appearance of the structure of gel T remained more homogeneous, while the pressurized gels exhibited more and larger cavities. © Springer-Verlag 1997.This research was financed by the Comision Interministerial de Ciencia y TecnologõÂa (CICyT) under project ALI-94-0786-C02-01.Peer Reviewe

zeitschrift für lebensmittel-untersuchung und -forschung. b, referate und lebensmittelrecht

Frozen storage of high-pressure- and heat-induced gels of blue whiting (Micromesistius poutassou) muscle: rheological, chemical and ultrastructure studies