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Alternaria  spp. fungi, characterized by a high tolerance to unfavorable environmental conditions, are one of the threats for foods of plant origin. The increasing incidence of diseases caused by a demanding lifestyle, and a higher social awareness of the role of a diet in maintaining health and good condition, results in the dynamically growing demand for natural protective measures that would be safe for consumers. Ellagitannins, i.e. a group of bio-active polyphenols, may constitute an alternative for chemical preservatives. Studies demonstrated that the raspberry ( Rubus idaeus  L.) ellagitannin formula limited the growth of  Alternaria alternata  0409. The minimal inhibitory concentration (MIC) was determined (0.156 mg/ml), along with the minimal fungicidal concentration (MFC) (0.312 mg/ml). The fungistatic (FA) activity and the ratio of linear growth (T) were also determined for the ellagitannin formula. A strong antimycotic activity of ellagitannins was demonstrated at the formula level of 0.1 mg/ml. Unfortunately, the activity was not maintained over time and after 9 days it was only 16.0%. For the ellagitannin formula, concentrations of 0.312 mg/ml (MFC) and 0.5 mg/ml (below the MFC value), a complete arrest of growth of  Alternaria alternata  0409 was observed, and it was maintained for 9 days. The antimycotic activity of the ellagitannin formula was also confirmed in food environment, with cottage cheese and cherry tomatoes used as the matrix. Results confirmed that ellagitannins from raspberry ( Rubus idaeus  L.) could be successfully used as a natural food preservative.

The antimycotic effect of ellagitannins from raspberry (Rubus idaeus L.) on Alternaria alternata ŁOCK 0409