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Natural isolates of Bacillus subtilis are known for their ability to producea large panel of bioactive compounds. Unfortunately, their recalcitrance toconventional molecular techniques limits their transcript studies. In thiswork, diYculties to isolate RNA attributed to the cell wall were overcome,Wnally authorising powerful RT-PCR's.

Resistance of the cell wall to degradation is a critical parameter for isolation of high quality RNA from natural isolates of Bacillus subtilis