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To the Editor: Type 1B diabetes or ketosis-prone atypical diabetes (KPAD) is a subtype of diabetes mostly described in patients of sub-Saharan African ancestry and characterized by insulin dependence at the time of presentation, without immunological markers seen in typical Type 1A diabetes (T1D), and frequently followed by absence of insulin requirements as in the case of Type 2 diabetes (T2D) [1, 2, 3]. The subsequent clinical course of KPAD, which could represent up to 12% of the sub-Saharan African diabetic population, is characterized by an alternance of near normoglycaemic remissions and relapses of insulin dependence without precipitating illness [3, 4]. Despite metabolic studies showing a beta-cell insulin secretory defect, no large genetic characterization has focused on this clinical subtype of diabetes. Mutations in the gene encoding the transcription factor hepatocyte nuclear factor (HNF)-1α impair glucose-stimulated insulin secretion and cause maturity onset diabetes of the young (MODY) [5]. It has been reported that the missense mutation Gly 574 (GGC) to Ser (AGC) (Gly574Ser) in HNF-1α was a marker of children-onset KPAD [6]. In a cohort of six auto-antibody negative African American children they found that four patients (67%) were carrying the heterozygote mutation compared to only 7% of the healthy non diabetic AfricanAmerican children [6]. In order to study the genetics of beta-cell dysfunction in the pathogenesis of KPAD, we gathered 80 unrelated black AfroCaribbean diabetic patients admitted to hospital with the typical diagnosis of KPAD (83% of sub-Saharan origin, 17% of Caribbean origin). All patients had new onset diabetes with acute signs of insulinopenia (ketosis or diabetic ketoacidosis and weight loss), without precipitating illness, and requiring insulin therapy. Means ± SD age was 39±9.5 years, islet cell antibodies and anti-glutamic acid decarboxylase 65 antibodies were negative in all patients. Of the patients 65 (81%) underwent near normoglycaemic remission of insulin dependence as previously defined [4] within one month following admission. Our initial objective was to assess whether the Gly574Ser mutation in the HNF-1α gene was also a marker of adult-onset KPAD. In all patients, we amplified the coding region of exon 9 of the HNF-1α gene by polymerase chain reaction (PCR) using specific primers (forward primer: 5′-CCAAGCAGGTAAGGTCCAGG-3′; reverse primer: 5′-CACAGTGACGGACAGCAACAG-3′). PCR products were purified using centrifugal filter devices (Microcon PCR, Millipore, Bedford, Mass., USA) and both strands were sequenced using the BigDye Terminator kit (Applied Biosystems, Foster City, Calif., USA) and analyzed on a ABI Prism 310 sequencer (Applied Biosystems). The cohort of KPAD patients was compared to a cohort of unrelated T2D patients, T1D patients, and normoglycaemic control subjects of the same Afro-Caribbean background. Informed consent was obtained in all subjects and the study was approved by the institutional review board of SaintLouis Hospital. We did not find any increased frequency of the Gly574Ser mutation in the KPAD cohort compared to the normoglycaemic control population [odds ratio 95% CI=0.85 (0.22–3.32)]. In addition, we did not find any association between the Gly574Ser polymorphism and the T2D or T1D diabetic cohorts. There was no difference between the Gly574Ser carriers and non-carriers respectively (Student’s t test or χ2) in the KPAD and T2D groups in terms of age of diabetes onset [KPAD (years): 33.9±6.9 vs 40.1±8.2; T2D: 40.2±8.8 vs 41.0±8.3; mean ± SD], existence of a family history of diabetes [KPAD: 40% vs 49%; T2D: 75% vs 71%], males/females ratio [KPAD: 80% vs 75%; T2D: 44% vs 50%] or BMI [KPAD (kg/m2): 25.7±4.6 vs 28.2±4.8; T2D: 27.8±6.3 vs 28.4±6; mean ± SD] (Table 1). This mutation has been exclusively reported in diabetic patients from African ancestry [6, 7, 8] and has never been observed in people from European or Asian descent. In all studies, there was no increased frequency of this mutation in the adult-onset T2D African-American population (4–7%) [6, 7] or sub-Saharan African populations (18%) [6] studied compared to control subjects. In a recent study a 16% frequency of the Gly574Ser mutation in a T2D population of 69 subjects Diabetologia (2003) 46:728–731

diabetologia

The polymorphism Gly574Ser in the transcription factor HNF-1α is not a marker of adult-onset ketosis-prone atypical diabetes in Afro-Caribbean patients