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Mentha cervina is a medicinal plant traditionallyused in Portugal in folk medicine, in different gastricdisorders and inflammations of the respiratory tract. Inorder to validate those traditional uses, M. cervina essentialoils (EOs) were characterized by GC and GC–MS and theirantimicrobial activity was tested against 23 bacterial strains(including multiresistant strains). The EOs were dominatedby the monoterpenes pulegone (52–75%), isomenthone(8–24%), limonene (4–6%), and menthone (1–2%). Theantibacterial activity of these EOs was compared to that ofthe main components standards. The most effective antibacterialactivity was expressed by the EOs against theGram-negative bacteria, Escherichia coli and Acinetobacterbaumanni, with MIC values of 1 mg/ml. The EOs complex mixtures were more active than the individualaromatic components supporting the hypothesis that theEOs antibacterial activity is a function of the synergisticeffect of their different aromatic components. These resultsshow the potential role of M. cervina EOs as antibacterialagents and validate the traditional use of this plant

Chemical composition and antibacterial activity of the essential oils from the medicinal plant Mentha cervina L. grown in Portugal