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To measure diamine oxidase (DAO) activity with high sensitivity in complex matrices like plasma or tissue extracts radioactive putrescine or horseradish peroxidase (HRP)/hydrogen peroxide (H 2 O 2 ) coupling must be used. The use of radioactive material should be avoided and HRP/H 2 O 2 coupling is compromised by antioxidants.

inflammation research

Simple, sensitive and specific quantification of diamine oxidase activity in complex matrices using newly discovered fluorophores derived from natural substrates