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We devise a new methodology to investigate terminal air spaces of lung. Micro-CT imaging and a computer guided technique for scanning and image fusion in confocal microscopy enable the acquisition of extended and highly resolved data volumes exhibiting respiratory units. Segmentation and the analysis of the topography of respiratory airways leads to a finite volume model suitable for computational physics within a computer lung model.

Morphological analysis of terminal air spaces by means of micro-CT and confocal microscopy and simulation within a functional model of lung