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BackgroundCytokines and cytokine antagonists modulate human immunodeficiency virus (HIV) replication in vitro and may be involved in HIV disease pathogenesis. An understanding of these cytokine networks may suggest novel treatment strategies for HIV-seropositive persons.Materials and MethodsU1 cells, a chronically infected promonocytic cell line, were stimulated with interleukin 1α (IL-1α), IL-1β or tumor necrosis factor (TNF) for 24 hr. The effects of these cytokines, and of anti-IL-1 receptor type 1 and type 2 (IL-1RI and II) antibody, IL-1 receptor antagonist (IL-1Ra), and recombinant human TNF binding protein type 1 (rhTBP-1, a form of TNF receptor p55), on HIV-1 replication, as measured by ELISA for HIV-1 p24 antigen, were determined. The effects of IL-1 and IL-1Ra on nuclear factor-κB (NF-κB) DNA binding activity, as measured by electrophoretic mobility shift assays, were also determined.ResultsIL-1α and IL-1β increased p24 antigen production in a concentration-dependent manner. IL-1Ra completely, and rhTBP-1 partially, suppressed IL-1-induced p24 antigen production. IL-1 increased NF-κB DNA binding activity and IL-1Ra blocked this effect. Since IL-1Ra blocks IL-1 from binding to both the IL-1RI and IL-1RII, monoclonal antibodies directed against each receptor were used to ascertain which IL-1R mediates IL-1-induced HIV-1 expression. Antibody to the IL-1RI reduced IL-1-induced p24 antigen production. Although anti-IL-1Rn antibody blocked the binding of 125IL-1-1α to U1 cells by 99%, this antibody did not affect IL-1-induced p24 antigen production. IL-1β enhanced TNFα-induced HIV expression when added before or simultaneously with TNFα.ConclusionsIL-1 induces HIV-1 expression (via the IL-1RI) and NF-κB activity in U1 cells. These effects are blocked by IL-1Ra and partially mediated by TNF. IL-1 enhances TNFα-induced HIV replication in U1 cells.

molecular medicine

Interleukin 1 Induces HIV-1 Expression in Chronically Infected U1 Cells: Blockade by Interleukin 1 Receptor Antagonist and Tumor Necrosis Factor Binding Protein Type 1