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Phytoplasma was detected inParthenium hysterophorus displaying symptoms of witches’-broom disease by direct and nested polymerase chain reaction using universal primers specific to 16S rRNA gene of phytoplasma. Sequence identities of 99% were found to several isolates of ‘Candidatus phytoplasma asteris’ (aster yellows, 16SrI group).

australasian plant disease notes

First report of phytoplasma ‘Candidatus phytoplasma asteris’ (16SrI) fromParthenium hysterophorus L. showing symptoms of virescence and witches’-broom in India