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The objective of this study was to obtain expanded blastocysts following intracytoplasmic sperm injection (ICSI) and Vero-cell co-culture, cryopreserve them at this stage, and transfer the frozen-thawed blastocysts to obtain pregnancies.

Human pregnancies after transfer of fresh (four-to eight-cell) versus frozen-thawed blastocysts resulting from intracytoplasmic sperm injection