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Several methods have been used hitherto to isolate amino acids from sea water. These methods are discussed in the first part of this paper, which is primarily concerned with the presentation of a new method, the desalting efficiency of which had been proved in a previous work by the author. In the newly described procedure, principal removal of inorganic salts is accomplished by ion retardation using resin Retardion 11 A8 (Dow Chemical Company, Midland, Mich., USA). The remaining ions and most of the organic compounds are adsorbed by ion exchange columns. It is thus possible to separate amino acids, carbohydrates and organic acids and obtain them in a salt-free solution. The new procedure very efficiently isolates some main groups of organic substances from one liter of sea water. Single components of these groups are then identified by paper chromatography. Employing the new method, several amino acids, carbohydrates, ascorbic acid and two organic acids could be isolated from sea water samples of the Deutsche Bucht (Southern North Sea) and from plankton cultures.

Isolation of dissolved organic substances from sea water with special reference to amino acids