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We have constructed a fast and easy method for the detection of O-gene in the ABO blood group system. Our method included allele specific amplification using polymerase chain reaction (PCR) and subsequent analysis with polyacrylamide gel electrophoresis (PAGE) and silver staining. O-gene product was observed on the gel in 25 DNA samples from group O phenotype and 20 DNA samples from group A or B (genotype AO or BO) persons. No product was found in 10 DNA samples from group AB phenotype.

O-gene detection by allele specific amplification in the ABO blood group system