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Summary  Human albumin prepared by two different procedures from the blood of seven normal persons and three diabetic subjects has been tested in two biological systemsin vitro, namely the glucose uptake of the rat diaphragm and the distribution of -amino-iso-butyric acid in the intact levator ani muscle of immature male rats.-Albumin fractions prepared by the trichloroacetic acid-ethanol procedure as it has been used byVallance-Owen et. al. (1958) showed in some cases an insulin antagonistic activity whereas other fractions did not show any insulin antagonism.-Albumin prepared as described byMichael (1962) constantly showed a high insulin-like activity but no antagonism. To some of these albumin fractions a specific anti-insulin serum was added, which partly reduced the insulin-like activity found in these preparations.-The two different assay systems used were found to give closely parallel results, which indicates that the source of conflicting findings probably resides in the steps of the procedure before the assay and not in the assay itself.-The results are discussed in connection to the theories put forward byVallance-Owen and co-workers.

Insulin-like activity and insulin-inhibitory effect of human albumin fractions studied with two biological test methods