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Summary Thirty strains of microorganisms (bacteria, yeasts, fungi and green algae) were tested as resting free cells for their ability to transform acetyldimethylphenylsilane (1) enantioselectively into (R)-(1-hydroxyethyl)dimethylphenylsilane [(R)-2]. The biotransformations were monitored by GC (packed OV-17 column) and the enantiomeric purities of the products isolated were determined by HPLC (cellulose triacetate column, UV detection). All microorganisms tested were found to reduce 1 enantioselectively to give (R)-2. Under the test conditions used, the yeastTrigonopsis variabilis (DSM 70714) was found to exhibit the highest specific activity (1.5 mg product x g cell wet mass-1 x min-1), whereas the highest enantioselectivities were observed for the bacteriaAcinetobacter calcoaceticus (ATCC 31012) (>95% ee),Brevibacterium species (ATCC 21860) (90% ee) andCorynebacterium dioxydans (ATCC 21766) (>95% ee), the yeastCandida humicola (DSM 70067) (90% ee), the fungusCunninghamella elegans (ATCC 26269) (94% ee), as well as the cyanobacteriumSynechococcus leopoliensis (94% ee). From the green algae tested,Chlamydomonas reinhardii showed the highest enantioselectivity (85% ee).

Enantioselective reduction of acetyldimethylphenylsilane: A screening with thirty strains of microorganisms