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Glycosides of glucose and lactose with di- and tetraethylene glycols, transformed into bifunctional (alcohol, ester) spacer molecules, have been synthesized. After deprotection, these spacer glycosides, containing a free carboxyl group, have been transformed efficiently into glycoconjugates using N,N,N',N'-tetramethyl(succinimido)uronium tetrafluoroborate (TSTU) for the formation of an active ester.

glycoconjugate journal

Synthesis of oligosaccharides with oligoethylene glycolspacers and their conversion into glycoconjugates usingN,N,N ? ,N ? -tetramethyl(succinimido)u roniu mtetrafluoroborate as coupling reagent