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ConclusionEstimation of aprotinin in plasma with the described amidolytic assay can be achieved within 70–80min and is, therefore, convenient as a bedside-control of high dose proteinase inhibitor therapy. Moreover, an ELISA for aprotinin developed simultaneously (Müller-Esterl et al. this issue) allows the detection of up to 0.2KIU/ml within 4h and is suitable, therefore, for special purposes, e.g. retrospective studies and low dose inhibitor therapy.

An enzymatic assay convenient for the control of aprotinin levels during proteinase inhibitor therapy