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of 0.6 ml of 105 mM K-phosphate buffer (pH 8.0), containing 0.1% bovine serum albumin, 0.5 mM ATP,,0.5 mM fructose1,6-bisphosphate, 0.5mM MgCI~ and 0.15mM CaCI 2 for 60 min at room temperature. After the addition of 0.1 ml of sensitized sheep erythrocytes [4.106 Ery preincubated with 0.005ml amboceptor (Behringwerke) for 60min at 20 C], incubation was continued for another 60min at 37 C. Unlysed erythrocytes were removed by centrifugation and lysis was measured at 416nm in the supernatants after addition of 0.1 ~tmole KCN.

Beiträge zur rechnerunterstützten Diagnostik II. Gruppierung biochemischer Schilddrüsenparameter ohne Berücksichtigung der klinischen Diagnose