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Summary  Several partially purified preparations of NSILA-S were tested for sulfation activity on rat and chicken embryo cartilage. A clearcut stimulation was obtained with as little as 0.1 U/ml and a near maximal effect with 10 U/ml. In no instance could sulfation activity be dissociated from NSILA-S. It is, therefore, likely that NSILA-S and sulfation activity are exerted by one and the same molecule. It is, however, compatible with our results and those from other laboratories that still other substances with sulfation activity besides NSILA-S may be present in serum. Morell's findings [7, 8] according to which NSILA-S stimulates growth and metabolism of chicken embryo fibroblasts and our results on NSILA-S stimulation of sulfate incorporation into cartilage point to a possible physiological role of NSILA-S as a growth factor.

diabetologia

Effects of partially purified preparations with nonsuppressible insulin-like activity (NSILA-S) on sulfate incorporation into rat and chicken cartilage