English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The VP1 gene of foot-and-mouth disease virus (serotype C1) has been cloned in Escherichia coli Clts cells, under the control of the bacteriophage lambda pL promoter. The expressed VP1 protein was complete and non-fused, and its molecular weight was indistinguishable from that of the VP1 obtained from virions. Cells harbouring the recombinant vectors exhibited symptoms of plasmid instability and toxicity and died in a few weeks even when never exposed to inducing conditions. A new plasmid clone in which a segment of the VP1 gene was fused with contiguous genes of the viral genome was very stable. The expressed partial VP1 protein contains the two major immunogenic domains of the virion. This system can be used as a tool to design an immunogenic VP1, and to explore possible synthetic vaccines against foot-and-mouth disease.

Molecular cloning and expression of the VP1 gene of foot-and-mouth disease virus C1 in E. coli: effect on bacterial cell viability