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A light-emitting diode-based photobioreactor (LED-based PBR) operated in a continuous perfusion mode with a perfusion rate of 3 to 6 reactor volumes a day supports high-density algal cultures, of cell concentrations up to 4·10 9 cells/mL, or 25 g/L. The oxygen production rate at its peak was 13 to 15 mmol/(L·h). Continuous medium perfusion allowed for long-term stable oxygen production, while oxygen production in batch mode ceased when stationary phase was reached

Continuous medium perfusion leads to long-term cell viability and oxygen production in high-density photobioreactors