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Specific binding sites for testosterone have been detected in three compartments of olfactory tissue from brown and rainbow trout. Binding of 3H-testosterone to the membrane fraction of olfactory tissue is of high affinity (KD = 0.5 - 1.9 nM) and limited capacity (NMAX = 30 - 60 fmol mg-1 protein). Binding is reversible, and is eliminated by protease treatment. The membrane binding site exhibits a high degree of ligand specificity; 11β-hydroxytestosterone, 11-ketotestosterone, 17α-hydroxyprogesterone, 17α, 20β-dihydroxy-4-pregnen-3-one, cortisol, and estradiol-17β all fail to displace testosterone at 20-fold excess while testosterone itself competes successfully. These attributes are consistent with the presence of specific steroid receptor proteins. Binding of testosterone within the cytosol is of moderate affinity (KD = 9.0 - 23.0 nM) and high capacity (Nmax = 0.5 - 2.9 pmol mg-1 protein) and is more readily displaced by a number of steroid competitors than is the case for the membrane site. The rate of association and dissociation of testosterone from the cytosolic binding site is markedly more rapid than the equivalent processes in the membrane fraction. Binding of testosterone to the nuclear extract is of high affinity (KD ~ 3.0 nM) and limited capacity (Nmax ~ 50 fmol mg-1 protein). \udThere are no substantial differences between species or between sexes in the affinity or capacity of testosterone-binding sites in nuclear extract or membrane fraction. However, cytosolic testosterone-binding sites are three- to four-fold more abundant in rainbow trout than in brown trout, and female rainbow trout have more cytosolic binding sites than male rainbow trout, but a lower affinity for testosterone than male sites. \udPreliminary evidence supports the involvement of the membrane-associated testosterone-binding site in olfactory processes. Rainbow trout display an EOG response to testosterone at a concentration (10-9 M) which is consistent with the equilibrium dissociation constant (KD) of the membrane-associated testosterone-binding site. Binding of 3H-testosterone to the membrane-associated site shows a pH dependancy which is comparable to the effects of pH on the EOG response to testosterone in intact fish. The attributes of the intracellular testosterone-binding sites are common to testosterone receptors in other fish tissues which are known androgen target tissues. This suggests that the development and/or function of salmonid olfactory tissue may be susceptible to influence by endogenous testosterone.\ud \u

Characterization of putative steroid receptors in the membrane, cytosol and nuclear fractions from the olfactory tissue of brown and rainbow trout