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The aim of this study is to propose a protocol allowing to merge informations acquired in vivo (MRI) and post mortem (histology). Departing from the 3-D histological volume and MRI, we first estimated a rigid transformation. Then, an elastic registration method using Free Form Deformation (FFD) was applied to correct the deformations due to the extraction of the brain from skull and the fixation. The final validation of the registration was based on a visual analysis of the matching of internal structures of the brain and the superimposition of cortical sulci.

Using 3D Non Rigid FFD-Based Method to Register post mortem 3D Histological Data and in vivo MRI of a Baboon Brain