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Determining kinetic rate constants is a highly relevant problem in biochemistry, so various methods have been designed to extract them from experimental data. Such methods have two main components: the experimental apparatus and the subsequent analysis, the latter often dependent on mathematical theory. Thus the theoretical approach taken influences the effectiveness of constant determination. A computational inverse problem approach is hereby presented, which does not merely give a single rough approximation of the sought constants, but is inherently capable of determining them from exact signals to arbitrary accuracy. This approach is thus not merely novel, but opens a whole new category of solution approaches in the field, enabled primarily by an efficient direct solver.

A Computational Resolution of the Inverse Problem of Kinetic Capillary Electrophoresis (KCE)