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Mitochondria are complex organelles, and their proteomics analysis requires acombination of techniques. The emphasis in this chapter is made first onmitochondria preparation from cultured mammalian cells, then on the separationof the mitochondrial proteins with two-dimensional electrophoresis (2DE),showing some adjustment over the classical techniques to improve resolution ofthe mitochondrial proteins. This covers both the protein solubilization, theelectrophoretic part per se, and the protein detection on the gels, which makesthe interface with the protein identification part relying on massspectrometry.

Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis