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A potential pseudoknot was found in the 3' untranslated region of the bovine coronavirus genome beginning 63 nt downstream from the stop codon of the N gene. Mutation analysis of the pseudoknot in a cloned defective interfering RNA indicated that this structural element is necessary for defective interfering RNA replication.

Evidence for a Pseudoknot in the 3′ Untranslated Region of the Bovine Coronavirus Genome