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We summarize our recent simulation progress of micromanipulation experiments on RNAs. We mainly consults with two important small RNAs unfolding experiments carried out by Bustamante group. Our results show that, in contrast to protein cases, using the single polymer elastic theory and the well-known RNA secondary structure free energy knowledge, we can successively simulate various behaviors of force unfolding RNAs under different experimental setups from equilibrium to far-from equilibrium.

Force Unfolding Single RNAs: From Equilibrium to Far-From Equilibrium