Animal Cell Biotechnology: Methods and Protocols

Immortalization Strategies for Mammalian Cells. Culturing Animal Cells in Fluidized Bed Reactors. Viral Evaluation of Animal Cell Lines Used In Biotechnology. Monitoring Animal Cell Growth and Productivity by Flow Cytometry. Adaptation of Mammalian Cells Grown in Serum Free Medium.Cytotoxicity Testing Using Cell Lines. Measuring the Folding Dynamics of Recombinant Proteins Secreted from Mammalian Cells.Hematopoietic Cells for Cellular and Gene Therapy: I. Basic Assay Techniques. Measurement of Cell-Culture Glycosidase Activity. Cytogenetic Characterization of Recombinant Cells. Hematopoietic Cells for Cellular and Gene Therapy: II. Expansion Protocols.Control of Proteolysis in Cell Culture. Measurement of Cell Death in Culture. GPI-Anchored Fusion Proteins. Harvesting GPI-Anchored Proteins From CHO Cells. Optimizing Gene Expression in Mammalian Cells.