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Fluorescence lifetime imaging creates a map of spatial differences in local lifetime. These lifetime differences can be correlated with local metabolic levels which makes this a valuable biomedical imaging modality. We examine the ability to localize a fluorescence signal from deeply embedded sites in highly scattering, turbid, tissue-like media. We show how a specific point-spread-like function may be used to quantify an isolated lifetime perturbation between two sites in tissue. This new minimally invasive method offers the opportunity to infer near-real-time estimates of metabolic activity.

Imaging Metabolism with Light: Quantifying Local Fluorescence Lifetime Perturbation in Tissue-like Turbid Media