Open AccessDetection of SARS‐CoV‐2 in two cats during the second wave of the COVID‐19 pandemic in France
Author(s) -
Fritz Matthieu,
Nesi Nicolas,
Denolly Solène,
Boson Bertrand,
Legros Vincent,
Rosolen Serge G.,
BriendMarchal Alexandra,
Ar Gouilh Meriadeg,
Leroy Eric M.
Publication year - 2022
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.638
Subject(s) - cats , serology , virology , covid-19 , antibody , pandemic , biology , immunoassay , medicine , immunology , pathology , outbreak , infectious disease (medical specialty) , disease
Although there are several reports in the literature of SARS‐CoV‐2 infection in cats, few SARS‐CoV‐2 sequences from infected cats have been published. In this study, SARS‐CoV‐2 infection was evaluated in two cats by clinical observation, molecular biology (qPCR and NGS), and serology (microsphere immunoassay and seroneutralization). Following the observation of symptomatic SARS‐CoV‐2 infection in two cats, infection status was confirmed by RT‐qPCR and, in one cat, serological analysis for antibodies against N‐protein and S‐protein, as well as neutralizing antibodies. Comparative analysis of five SARS‐CoV‐2 sequence fragments obtained from one of the cats showed that this infection was not with one of the three recently emerged variants of SARS‐CoV‐2. This study provides additional information on the clinical, molecular, and serological aspects of SARS‐CoV‐2 infection in cats.