Open AccessCloning and Functional Analysis of Erythropoietin‐, Interleukin‐3‐and Thrombopoietin‐Inducible Genes
Author(s) -
D'Andrea Alan D.,
Zhu Yuan
Publication year - 1996
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.5530140710
Subject(s) - biology , thrombopoietin , gene , receptor , cloning (programming) , clone (java method) , cytokine receptor , erythropoietin , microbiology and biotechnology , signal transduction , genetics , stem cell , haematopoiesis , computer science , programming language
The receptor for thrombopoietin (TPO) is a member of the cytokine receptor superfamily. This superfamily also includes the receptors for erythropoietin (EPO), interleukin 3 (IL‐3), GM‐CSF and several other cytokines. Stimulation of cytokine receptors with their cognate ligands results in the activation of multiple signal transduction pathways and ultimately in the induction of new genes. The cloning of these specific genes provides one approach for analyzing cytokine‐specific responses. In the current study, we have designed a strategy for isolating inducible genes. While the strategy has been used to identify EPO‐specific and IL‐3‐specific inducible genes, the strategy can be extended to clone TPO‐inducible genes.