Open AccessSingle Cell Phenotyping Reveals Heterogeneity Among Hematopoietic Stem Cells Following Infection
Author(s) -
MacLean Adam L.,
Smith Maia A.,
Liepe Juliane,
Sim Aaron,
Khorshed Reema,
Rashidi Narges M.,
Scherf Nico,
Krinner Axel,
Roeder Ingo,
Lo Celso Cristina,
Stumpf Michael P. H.
Publication year - 2017
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.2692
Subject(s) - biology , niche , stem cell , haematopoiesis , bone marrow , motility , hematopoietic stem cell , microbiology and biotechnology , immunology , dynamics (music) , stem cell niche , inflammation , progenitor cell , ecology , physics , acoustics
Abstract The hematopoietic stem cell (HSC) niche provides essential microenvironmental cues for the production and maintenance of HSCs within the bone marrow. During inflammation, hematopoietic dynamics are perturbed, but it is not known whether changes to the HSC–niche interaction occur as a result. We visualize HSCs directly in vivo, enabling detailed analysis of the 3D niche dynamics and migration patterns in murine bone marrow following Trichinella spiralis infection. Spatial statistical analysis of these HSC trajectories reveals two distinct modes of HSC behavior: (a) a pattern of revisiting previously explored space and (b) a pattern of exploring new space. Whereas HSCs from control donors predominantly follow pattern (a), those from infected mice adopt both strategies. Using detailed computational analyses of cell migration tracks and life‐history theory, we show that the increased motility of HSCs following infection can, perhaps counterintuitively, enable mice to cope better in deteriorating HSC–niche microenvironments following infection. S tem C ells 2017;35:2292–2304