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We have demonstrated that human neonatal cardiosphere‐derived cells (CDCs) derived from the young are more regenerative due to their robust secretome. However, it is unclear how the decompensated pediatric heart impacts the functional activity of their CDCs. Our aim was to characterize the potency of pediatric CDCs derived from normal functioning myocardium of control heart disease (CHD) patients to those generated from age‐matched end stage heart failure (ESHF) patients and to determine the mechanisms involved. ESHF‐derived CDCs contained a higher number of c‐kit + , Islet‐1 + , and Sca‐1 +  cells. When transplanted into an infarcted rodent model, ESHF‐derived CDCs significantly demonstrated higher restoration of ventricular function, prevented adverse remodeling, and enhanced angiogenesis when compared with CHD patients. The superior functional recovery of the ESHF‐derived CDCs was mediated in part by increased SDF‐1α and VEGF‐A secretion resulting in augmented recruitment of endogenous stem cells and proliferation of cardiomyocytes. We determined the mechanism is due to the secretome directed by the heat shock response (HSR), which is supported by three lines of evidence. First, gain of function studies demonstrated that increased HSR induced the lower functioning CHD‐derived CDCs to significantly restore myocardial function. Second, loss‐of function studies targeting the HSR impaired the ability of the ESHF‐derived CDCs to functionally recover the injured myocardium. Finally, the native ESHF myocardium had an increased number of c‐kit +  cardiac stem cells. These findings suggest that the HSR enhances the functional activity of ESHF‐derived CDCs by increasing their secretome activity, notably SDF‐1α and VEGF‐A. S tem  C ells   2015;33:1213–1229

stem cells

Cardiosphere‐Derived Cells from Pediatric End‐Stage Heart Failure Patients Have Enhanced Functional Activity Due to the Heat Shock Response Regulating the Secretome