Open AccessAutoinduction of Protein Expression
Author(s) -
Fox Brian G.,
Blommel Paul G.
Publication year - 2009
Publication title -
current protocols in protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.409
H-Index - 32
eISSN - 1934-3663
pISSN - 1934-3655
DOI - 10.1002/0471140864.ps0523s56
Subject(s) - escherichia coli , protein expression , computational biology , protocol (science) , chemistry , biochemistry , biology , medicine , gene , alternative medicine , pathology
This unit contains protocols for the use of lactose‐derived autoinduction in Escherichia coli . The protocols allow for reproducible expression trials to be undertaken with minimal user intervention. A basic protocol covers production of unlabeled proteins for functional studies. Alternate protocols for selenomethionine labeling for X‐ray structural studies, and multi‐well plate growth for screening and optimization are also included. Curr. Protoc. Protein Sci . 56:5.23.1‐5.23.18. © 2009 by John Wiley & Sons, Inc.