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We show that the static force spectroscopy curve is significantly modifieddue to presence of intrinsic cantilever instability. This instability acts intandem with such instabilities like water bridge or molecular bond rupture andmakes the static force spectroscopy curve (including "jump-off-contact")dependent on the step-size of the movement of sample stage. A model has beenproposed to explain the data. This has been further validated by applying anelectric field between tip and substrate which modifies the tip-substrateinteraction.

Effect of intrinsic instability of cantilevers on static mode Atomic Force Spectroscopy