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We show that single walled carbon nanotubes with different isotopecompositions exhibit distinct Raman Gband peaks and can be used for multiplexedmulti-color Raman imaging of biological systems. Cancer cells with specificreceptors are selectively labeled with 3 differently colored SWNTs conjugatedwith various targeting ligands including Herceptin, anti-Her2, Erbitux,anti-Her1, and RGD peptide, allowing for multi-color Raman imaging of cells ina multiplexed manner. SWNT Raman signals are highly robust againstphoto-bleaching, allowing long term imaging and tracking. With narrow peakfeatures, SWNT Raman signals are easily differentiated from theauto-fluorescence background. The SWNT Raman excitation and scattering photonsare in the near-infrared region, which is the most transparent optical windowfor biological systems in vitro and in vivo. Thus, SWNTs are novel Raman tagspromising for multiplexed biological detection and imaging.

Multiplexed Multi-Color Raman Imaging of Live Cells with Isotopically Modified Single Walled Carbon Nanotubes