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Heavy metal contamination of agricultural land is partly responsible forlimiting crop productivity. Cd$^{2+}$ is known as a non-essentiel HM that canbe harmful to plants even at low concentrations. Brassica juncea (L.) is ableto accumulate more than 400 $\mu$g.g$^{-1}$ D.W. in the shoot, a physiologicaltrait which may be exploited for the phytoremediation of contaminated soils andwaters. . The application of 75 $\mu$M CdCl$_2$ for three days does not showany effect in the B. juncea growth parameters (F.W. and D.W.) whatever the typeof plantlets. This application decreases also the contents of chlorophyll a,carotenoids and Chl a/b ratio (2.26) for plantlets regenerated in the absenceof CdCl$_2$ but not those of plantlets regenerated in its presence. Roots havethe highest contents (3071; 1544 $\mu$g.g$^{-1}$ D.W.) followed by stems (850;687$\mu$g.g$^{-1}$ D.W.) and leaves (463; 264$\mu$g.g$^{-1}$ D.W.)respectively. In our conditions, we suggest that the low accumulation in theplantlets regenerated in the presence of CdCl$_2$ by the means of in-vitroregeneration technology is still benefical, to some extent, for thephytoextraction process and seems to be an interesting technology that allowsthe cultivation of these plantlets in contaminated soils with low accumulationof metal in their shoots and probably in their seeds used in many foodtechnologies.

Potential Phytoextraction with in-vitro regenerated plantlets of  Brassica juncea (L.) Czern. in presence of CdCl$_2$: Cadmium accumulation and  physiological parameter measurement