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The binding constant of copper(II) ions to C-PC were determined at differentionic strengths from binding isotherms by equilibrium dialysis and flame atomicabsorption spectroscopy. Fluorescence and absorbtion spectroscopy providesinsight of metal-C-phycocyanin interactions. Fluorescence measurementsdemonstrate C-PC quenching of heavy metal ions emission intensities.Stern-Volmer quenching constants were obtained from the linear quenching plots.Blue shifts in the fluorescence spectra were observed during metal binding toC-PC. It was shown, that between bound metal ions in C-PC there exists positivecooperativity.

Interacion of Heavy Metal Ions with C-Phycocyanin: Binding Isotherms and Cooperative Effects