Open AccessQuantification of Impurity-G Content in Levetiracetam Injection (100 mg/ml) by Using RP-HPLC Technique
Author(s) -
Syed Mastan Ali,
P. Bharath,
V. Ramakrishna,
D. Ramachandran
Publication year - 2022
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2022/v34i28b36035
Subject(s) - impurity , chromatography , high performance liquid chromatography , levetiracetam , detection limit , analytical chemistry (journal) , acetonitrile , chemistry , materials science , medicine , organic chemistry , psychiatry , epilepsy
Extremely responsive technique for the quantification of impurity-G content in Levetiracetam samples by RP-HPLC method has been presented in the here manuscript. Quantification of impurity-G content in Levetiracetam sample by HPLC with UV Detector. Impurity-G was concluded by RP-HPLC technique by waters X-Terra MS-C18 (250 x 4.6mm, 5µm) column as motionless segment. Column oven preserved 45°C and taster cooler temperature 5°C, injection quantity 50µL, flow rate was 1.0 mL/min, impurity-G was detected using UV detector at the wavelength of 200nm and run time was 30 minutes. The movable segment utilized pH 3.0 buffer and acetonitrile in the proportion of (850:150 v/v) isocratic mode. The technique legalization has been carried as per International Conference on Harmonization guidelines (ICH). Limit of quantitation (LOQ) was found 0.4122 µg/mL for impurity-G.