Open AccessPhytochemical Analysis and Antioxidants effects of Methanol Stem Bark Extract of Eucalyptus Camaldulensis in Wister Albino Rats
Author(s) -
Jude Nwaogu,
Babangida Abubakar,
Mustapha Sahabi Aliyu
Publication year - 2021
Publication title -
journal of complementary and alternative medical research
Language(s) - English
Resource type - Journals
ISSN - 2456-6276
DOI - 10.9734/jocamr/2021/v14i330248
Subject(s) - phytochemical , eucalyptus camaldulensis , oxidative stress , terpenoid , antioxidant , traditional medicine , distilled water , carbon tetrachloride , vitamin c , chemistry , vitamin e , ccl4 , medicine , food science , biology , biochemistry , botany , eucalyptus , chromatography , organic chemistry
Aim: The aim is to evaluate the antioxidant potentials of Eucalyptus camaldulensis methanol stem bark extract (ECMSBE) on Wister albino rats.
Methodology: The phytochemical screening of ECMSBE was conducted using standard methods. A total of 36 albino rats were used for the antioxidant studies. The rats were divided into six (6) groups of six rats. Control group received distilled water orally at 2ml/kg. Oxidative stress was induced in groups 2 to group 6 CCl4 (1ml/kg, s.c) at every 72hrs for 10 days. Group 2 was untreated while groups 3 – 6 received doses of 50, 100, 150 and 200mg/kg of ECMSBE respectively. On the 11th day, the rats were sacrificed and the liver was removed and homogenised and oxidative stress parameters were determined.
Results: Phytochemical analysis of ECMSBE revealed the presence of saponins, flavonoids, tannins, phenols, glycosides, steroids, terpenoids and resins. There was no significant difference (P .05) in the levels of SOD, CAT and GPx between the group induced with CCl4 only and the group treated with ECMSBE (50mg/kg), however, it these concentrations were significantly higher (P .05) when compared the group that was treated with ECMSBE (200mg/kg)
Conclusion: The result suggest that the extract of E. camaldulensis possessed antioxidant properties which can be used as effective protecting agents against oxidative stress and other diseases.