Open AccessEvaluation the Biological Activity of Nano- Gentamicin Prepared and Characterization of Nanoparticles
Author(s) -
Rana Kamal,
Quraish A. Kahdhum,
Awatif I. Mohammed,
Ahmed J. Essa,
Maan A. Abd elhamid,
Essraa A. Mohamad
Publication year - 2021
Publication title -
iraqi journal of industrial research
Language(s) - English
Resource type - Journals
ISSN - 2788-712X
DOI - 10.53523/ijoirvol8i2id79
Subject(s) - gentamicin , antibacterial activity , gentamicin sulfate , chemistry , nuclear chemistry , chloroform , scanning electron microscope , nanoparticle , acetone , staphylococcus epidermidis , gram positive bacteria , aqueous solution , gram negative bacteria , gram , bacteria , chromatography , materials science , nanotechnology , escherichia coli , antimicrobial , antibiotics , organic chemistry , staphylococcus aureus , biochemistry , biology , composite material , genetics , gene
Gentamycin is a broad-spectrum antibacterial against Gram-positive and Gram-negative bacteria. Gentamycin Sulfate is a white powder that is freely soluble in water and insoluble in alcohol, acetone, chloroform and ether. The study aims to determine the characterization and the biological activity of gentamicin in the Nano-form prepared by Sol-gel application using an ultrasound device by aqueous solution at temperature of 80°. Nano gentamycin examined by Scanning electron microscopy (SEM), transmission (TEM) and the antibacterial effect test of both Nano and stander gentamycin were analyzed. The results showing that the average size of gentamycin nanoparticles is 68.51 nanometers with homogeneous distribution. The antibacterial effect of Nano gentamycin take a wide inhibition range of Gram-positive and Gram-negative bacteria compared to the standard substance, the inhibition diameters are for E. coli (29 mm) and for St. epidermidis (27 mm). In the other side the inhibitory ability for standard gentamicin for St. epidrmidis and E. coli were 18 mm and 20 mm, respectively.