Open AccessExpression of Heat Shock Protein HSP90 in Genomic-DNA of Chickpea (Cicer arietinum L.) Callus by Heat Shock Treatment
Author(s) -
Anwaar F AL-Taee,
AUTHOR_ID,
Jamella H Rasheed,
AUTHOR_ID
Publication year - 2022
Publication title -
al-mağallaẗ al-akādīmiyyaẗ li-l-abḥāṯ wa-al-našr al-ʿilmī
Language(s) - English
Resource type - Journals
ISSN - 2706-6495
DOI - 10.52132/ajrsp.e.2022.33.6
Subject(s) - callus , heat shock protein , abiotic component , thermal shock , gene , hsp90 , biology , transcription (linguistics) , shock (circulatory) , heat shock factor , microbiology and biotechnology , hsp70 , gene expression , transcription factor , horticulture , botany , biochemistry , materials science , medicine , paleontology , linguistics , philosophy , composite material
This study was able to detect of the expression activity of heat shock proteins HSP90 and heat transcription factors HSFs for the first time in callus cultures of chickpea, Cicer arietinum L., that exposed to abiotic shocks, grown on MS medium supplemented with 1.0 mg L-1 naphthalene acetic acid (NAA) and 2.0 mg L-1 benzyl adenine (BA). Heat shock proteins HSPs were constructed for increase of withstand long-term physical shocks, and production of resistant to heat chickpeas plants, this shock was enhancement of tolerance of chickpea callus to abiotic stresses (high - temperatures). Results enhanced the ability of chickpea callus to abiotic stresses bearing and induce of HSF genes to heat shock proteins HSP90 production quickly to removing denatured proteins, avoid apoptosis, thus, supporting tolerance to the sudden action of these shocks. Expression activity of heat shock genes and transcription factors by determined based on polymerase chain reaction qPCR, that explained the gene activity increasing at shocks intensity increased