Open AccessSimultaneous determination of pyrrolizidine alkaloids in food supplements of herbal origin using liquid chromatography tandem mass spectrometry
Author(s) -
Sơn Trần Cao,
Tien Bui Cao,
Thao Dang Phuong,
Truong Nguyen Xuan,
To Quynh Cung Thi,
Hồng Hảo Lê Thị
Publication year - 2019
Publication title -
kiểm nghiệm và an toàn thực phẩm/kiểm nghiệm và an toàn thực phẩm (online)
Language(s) - English
Resource type - Journals
eISSN - 2734-9152
pISSN - 2615-9252
DOI - 10.47866/2615-9252/vjfc.58
Subject(s) - pyrrolizidine , chromatography , repeatability , chemistry , tandem mass spectrometry , mass spectrometry , liquid chromatography–mass spectrometry , pyrrolizidine alkaloid , stereochemistry
A simple and accurate method has been developed for the simultaneous determination of four pyrrolizidine alkaloids (PAs) including echimidine, senecionine, jacobine and intermidine in food supplements of plant origin. The instrumental conditions including the use of TSK-Gel column for the separation and an MS/MS detector in positive ESI source, multi-reactive monitoring (MRM) mode for quantitation were optimized. The method has fully validated and meets the performance requirement of AOAC with good specificity. The linearity varies from 1.0 µg/kg to 40 µg/kg, the LOD was of 0.3 µg/kg; the repeatability and recovery range from 3.6% to 7.5% and 88.7% to 117%, respectively. The method was applied to determine PAs in 90 food supplement samples, of which 26 samples (28.8%) contain PAs.