Open AccessIsolation and identification of Bifidobacterium spp. from infant intestinal tract
Author(s) -
Mến Đỗ Thị,
Lệ Phạm Thị,
Tuấn Trần Văn,
Tuyết Lan Ninh Thị,
Minh Huyền Nguyễn Thị
Publication year - 2020
Publication title -
kiểm nghiệm và an toàn thực phẩm/kiểm nghiệm và an toàn thực phẩm (online)
Language(s) - English
Resource type - Journals
eISSN - 2734-9152
pISSN - 2615-9252
DOI - 10.47866/2615-9252/vjfc.104
Subject(s) - bifidobacterium , bifidobacterium longum , bacteria , probiotic , biology , isolation (microbiology) , food science , microbiology and biotechnology , feces , lactobacillus , genetics
As one of probiotic bacteria, Bifidobacterium is supplement in food industry such as in milk and yogurt. The advantageous impact of these bacteria for human health was reported. However, in Vietnam, supplement of these bacteria as probiotic source to the food was not much in food industry. In our experiment, Bifidobacterium are isolated from a feces sample of 7 days-child which only had mother milk. The bacterial isolated from infant intestinal tract was Bifidobacterium infantis (B. infantis, closest to Bifidobacterium longum ssp. infantis strain Bi-26 with gene bank accession CP054425.1). The techniques to identify these bacteria were MALDI Biotyper, PCR and sequencing. This experiment is original for further study the characters of these bacteria to apply them in our daily life.