Open AccessBiosynthesis, Characterization, Antibacterial Activity and Anticancer Effect of Silver Nanoparticles Using Anethum Graveolens Leaf Extract
Publication year - 2020
Publication title -
journal of environmental treatment techniques
Language(s) - English
Resource type - Journals
ISSN - 2309-1185
DOI - 10.47277/jett/8(4)1629
Subject(s) - anethum graveolens , enterococcus faecalis , antibacterial activity , silver nanoparticle , antimicrobial , chemistry , minimum inhibitory concentration , escherichia coli , nanoparticle , minimum bactericidal concentration , nuclear chemistry , agar diffusion test , antibiotics , nanotechnology , bacteria , microbiology and biotechnology , combinatorial chemistry , materials science , biology , biochemistry , organic chemistry , botany , genetics , gene
Nanoparticles are used in various fields of science, especially medicine. Advent of nanotechnology has led to significant development in disease diagnosis, treatment and drug delivery. Silver nanoparticles (Ag-NPs) play an important role in medical application, which makes them a viable alternative to common antibiotics. Amongst various methods, synthesis of Ag-NPs via green method has the advantage of being cost effective with no toxic agent. In this study, Spherical shape Ag-NPs with average size of 30 nm were synthetized using Anethum graveolens leaf extract as a green, cost-effective, non-toxic and environment-friendly source. Transmission electron microscopy (TEM), particle size analysis (PSA) and Fourier transform infrared (FT-IR) were performed to characterize synthesized Ag-NPs. The antibacterial activity of the synthetized Ag-NPs was evaluated against gram positive and negative bacterial pathogens. The minimum inhibitory concentration (MIC) at different concentrations of Ag-NPs were used to evaluate their antibacterial properties against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa pathogens. The results exhibited a desirable antibacterial property of Ag-NPs, suggested its usage as putative antibacterial agents. Moreover, the anticancer effect of green synthesized Ag-NPs was evaluated against MCF-7 lines and results showed that the cell viability is depended on the concentration of Ag-NPs. In short, this method provides a simple, cost effective and eco-friendly way to synthesis Ag-NPs which can be used as a suitable alternative to common antibiotics that use hazardous chemical agents, additionally, with anticancer effects against MCF-7 cells.