Open AccessApplication of polymerase chain reaction (PCR) and PCR based restriction fragment length polymorphism for detection and identification of dermatophytes from dermatological specimens
Author(s) -
R Bagyalakshmi,
B Senthilvelan,
K Lily Therese,
S Murugusundram,
Madhavan Hn
Publication year - 2008
Publication title -
indian journal of dermatology/indian journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.395
H-Index - 36
eISSN - 1998-3611
pISSN - 0019-5154
DOI - 10.4103/0019-5154.39735
Subject(s) - restriction fragment length polymorphism , polymerase chain reaction , internal transcribed spacer , biology , dermatophyte , microbiology and biotechnology , gene , ribosomal rna , genetics
To develop and optimize polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) targeting 18S rDNA and internal transcribed spacer (ITS) region of fungi for rapid detection and identification of dermatophytes.